WebPlasmid pTRE-EGFP from Dr. Hyungbae Kwon's lab contains the insert tetO-EGFP and is published in Nat Methods. 2024 May;14(5):495-503. doi: 10.1038/nmeth.4234. Epub 2024 … WebBbsI gradually loses activity when stored at -20°C. Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. SmaI can be used at 37°C for …
pTRE3G Sequence and Map - SnapGene
WebAll plasmid-based versions of the Tet-On 3G System contain the following seven components: 10 μg control plasmid expressing luciferase under the control of a TRE3G promoter (either pTRE-Luc or pTRE-BI-Luc) 2 μg Linear Hygromycin Marker (also sold separately as Cat. # 631625) 2 μg Linear Puromycin Marker (also sold separately as Cat. … WebDec 7, 2015 · The pTRE-Myc Vector contains an MCS downstream of the TetresponsiveP hCMV*-1promoter. cDNAs or genes inserted in the MCS will be responsive to either thetTA or rtTA regulatory protein when expressed by a separate construct in the Tet-Off or Tet-Onsystems, respectively. The P hCMV*-1promoter contains the Tet-responsive element … optical warehouse of greenpoint brooklyn ny
Addgene: pTRE-EGFP
WebOverview. Winchester Varmint X 223 Remington 40gr PTRE Rifle Ammo - 20 Rounds - Designed specifically for hunting varmints, the Winchester Varmint X line of ammunition features a polymer tip for maximum flight stabilization and penetration. The alloy jacket and lead core are designed to rapidly fragment, producing devastating results. WebThe Tet-Dual transactivator expression vectors constitutively express either the Tet-On Advanced or Tet-Off Advanced protein, and the fluorescent protein, ZsGreen1 (Panel A). pTRE-Dual2 is a Tet-regulated inducible expression vector that coexpresses mCherry and a second gene that is cloned in the multiple cloning site (MCS) (Panel B). pTRE ... Webאתר טיולי מציע מאות מסלולי טיול למשפחות, לקבוצות, לזוגות וליחידים - הסברים, מפות, פורום טיולים פעיל וכל מה שחשוב לדעת לפני שיוצאים לטייל, במרחק לחיצה optical warehouse of greenpoint