Orc2 antibody drosophila
WebOct 8, 2010 · IP with Orc2 antibody followed by immunoblot with ORCA polyclonal antibody (pAb) showed the association of Orc2 with endogenous ORCA (molecular weight of ORCA ∼70kDa; Figure 1 D). Finally, immunoprecipitation with ORCA pAb in human cells clearly demonstrated the association of ORCA with ORC subunits ( Figure 1 E). WebApr 1, 1999 · We isolated mutations in Drosophila E2F and DP that affect chorion gene amplification and ORC2 localization in the follicle cells. In the follicle cells of the ovary, the ORC2 protein is localized throughout the follicle cell nuclei when they are undergoing polyploid genomic replication, and its levels appear constant in both S and G phases.
Orc2 antibody drosophila
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WebDec 30, 2005 · The Drosophila k43 gene, which encodes the orc2 subunit, has been identified in two independent screens: a late-larval lethal mutation with small or missing … WebNational Center for Biotechnology Information
WebJul 30, 2008 · We showed previously by coimmunoprecipitation that Drosophila Orc1 and Orc2 proteins interact with the dDP/dE2F1/Rbf1 complex in ovarian extracts, and we … WebDrosophila provides a powerful model for understanding replication control in metazoans. ... The anti-ORC2 antibodies were provided by Richard Austin and Steve Bell and used at a dilution of 1:2500. Anti-DUP antibodies were used at a dilution of 1:1000. Microscopy for fluorescently labeled embryos and ovaries was done using either a Biorad ...
WebWe isolated mutations in Drosophila E2F and DP that affect chorion gene amplification and ORC2 localization in the follicle cells. In the follicle cells of the ovary, the ORC2 protein is … WebApr 13, 2024 · This study shows that Drosophila germ granules, which initially protect RNAs, subsequently become sites of selective mRNA degradation; this plasticity supports germ cell development. ... 50 μg/mL tRNA), embryos were incubated with primary antibody in PBHT overnight at 4°C with rocking. Primary antibodies used: 1:250 rabbit anti-CCR4 , 1:250 ...
WebNov 2, 2011 · We thank I. Chesnokov and the Bloomington Drosophila Stock Center (Bloomington, IN) for flies, S. Bell and M. Botchan for α-Orc2 antibodies, J. Powers of the Indiana Light Microscopy Imaging Center (Bloomington, IN) for helpful advice, and M. Lilly and members of the Calvi lab for advice and comments on the manuscript. We thank M. …
WebMay 1, 2004 · Antibody to the origin recognition complex subunit 2 (ORC2)recognizes large foci that localize to the endogenous chorion gene loci and to active transgenic constructs … hilfen synonymWebDrosophila Myb recognizes a highly conserved DNA sequence10,11, but the specific binding properties of the glycerol gradient fractions might be more complex than that of … smarsh wikipediaWebPreparation of the ORC2 antibodies. The Drosophila ORC2 coding sequence was PCR amplified from a 4- to 8-hr embryonic cDNA library (Brown and Kafatos 1988). The PCR product was subcloned into pCYB2 (New England Biolabs) usingNdeI and SmaI sites engineered into the termini of the PCR product. DmORC2 protein was expressed in … smarsh webmail owaWebOct 9, 2024 · Meier-Gorlin syndrome (MGS) is a rare autosomal recessive disorder characterized by microtia, primordial dwarfism, small ears and skeletal abnormalities. … smarsh whatsappWebThe origin-recognition complex (ORC) has an essential role in defining DNA replication origins [1] and in chromosome segregation [2, 3]. Recent studies in Drosophila orc2 mutants [4–6], and in human cells depleted of ORC2 [3], have suggested that this factor is also implicated in mitotic chromosome assembly. We asked whether ORC was required for M … smarsh zoominfoWebJul 30, 2008 · We showed previously by coimmunoprecipitation that Drosophila Orc1 and Orc2 proteins interact with the dDP/dE2F1/Rbf1 complex in ovarian extracts, and we wished to further characterize this interaction . ... Anti-HA (Sigma), Anti-ORC2, and anti-dE2F1 antibodies were used at 1∶100 dilutions. Anti- RBF2 was used at 1∶1 dilutions. Anti-V5 ... hilfenhaus cricinfoWebJun 24, 2004 · The centrosomes of HeLa cells arrested in the aberrant mitotic state failed to label with anti-Orc2 antibodies, suggesting that the Orc2 centrosome labeling was specific (Supplementary Figure S10B). ... Mutations in Drosophila Orc2 also disrupt HP1 association with heterochromatin (Huang et al, 1998). hilferenpass