Its5f
WebThe ITS primer pair used were: forward: ITS5F (5’-GGAAGTAAAAGTCGTAACAAGG–3’) and reverse: ITS4R (5ʹCTCCTCCGCTTATTGATATGC−3’) (White et al. Citation 1990). The PCR reaction consisted of template DNA, 2.5 μM forward and reverse primers, 25 mM MgCl 2, 10 mM dNTPs, Taq DNA polymerase, 10 mM buffer (Thermo Scientific, USA). Web15 jan. 2024 · The Yellow River Delta is the youngest and largest estuarine wetland in temperate China and harbors a high biodiversity level. The fine-scale field investigation was performed in a P. australis wetland of the Yellow River Delta in October 2024 (Fig. 1).To avoid the same genotypes, a total of 20 shoots of P. australis were sampled along a 1 …
Its5f
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Web29 mrt. 2024 · The effects of hydrogen sulfide (H2S) on diabetic metabolic disorders are still controversial, and the mechanisms underlying these effects remain largely unknown. This study was conducted to investigate the potential relationship between the gut microbiota and the improvement of diabetic metabolic disorders by exogenous H2S in obese db/db … Web428 Y. Zhu et al. 1 3 soilisreleasedintotheactivelayer[19].Astheperiodof meltingincreased,however,long‑termrivererosionandthe ...
Web10 jan. 2024 · The PCR primers used for detecting the fungal 18S rDNA gene were ITS5F (5′-GGAAGTAAAAGTCGTAACAAGG-3′) and ITS2R (5′GCTGCGTTCTTCATCGATGC … WebNational Center for Biotechnology Information
WebCN107604089A CN202411002452.3A CN202411002452A CN107604089A CN 107604089 A CN107604089 A CN 107604089A CN 202411002452 A CN202411002452 A CN 202411002452A CN 107604089 A CN107604089 A CN 107604089A Authority CN China Prior art keywords sequence dendrobium stem species dna Prior art date 2024-10-24 … Web12 aug. 2024 · This study is designed to understand the community structure and diversity of fungi in the rhizosphere soil of grape. As the sample for this study, the rhizosphere soil of …
Web5 sep. 2024 · ITS1 region of fungal ribosomal DNA (rDNA) was amplified with ITS5F(5’-GGAAGTAAAAGTCGTAACAAGG-3’) and ITS2R (5’-GCTGCGTTCTTCATCGATGC …
Web9 sep. 2024 · In comparison with ectomycorrhizal (EM) tree species, arbuscular mycorrhizal (AM) trees have different litter quality and nitrogen cycle modes, which may affect mycorrhizal colonization and the community composition and diversity. However, available studies addressing the mycorrhizal fungal colonization rate, diversity and community … reign this inWeb30 mrt. 2024 · AimsIn garlic cultivation, long-time monoculture has resulted in continuous-cropping obstacles. However, the cause has not been studied to date.MethodsWe analyzed soils from garlic fields in Pengzhou, China, to determine continuous-cropping obstacle related changes in soil physicochemical properties and enzyme activities, and in the … reign the towelWeb19 sep. 2024 · In accordance with our previous study , 338F/806R and ITS5F/ITS1R primers were used for the PCR amplification of the bacterial 16S rRNA V3-V4 region and the fungal ITS1 region, respectively, and then, high-throughput sequencing (PE, 2 × 250 bp) of the PCR products was performed at Shanghai Personal Biotechnology Co., Ltd. (Shanghai, … procter and gamble pakistan jobsWeb1 aug. 2024 · The 18 S rDNA gene was amplified in the ITS1–5 F region with the primers ITS5F (5′-GGAAGTAAAAGTCGTAACAAGG-3′) and ITS2R (5′-GCTGCGTTCTTCATCGATGC-3′). Subsequently, TruSeq® DNA PCR-free Sample Preparation Kit (Illumina, USA) was used to construct bacterial and fungal libraries for all … procter and gamble oxnard plantWeb25 sep. 2009 · The amplification profile consisted of an initial denaturation step of 94°C for 5 min, followed by 40 cycles of 94°C for 1 min, 48°C for 1 min, 72°C for 1 min, and a final elongation step of 72°C for 5 min. Amplified fragments were purified using a MinElute PCR Purification Kit (Qiagen), and the sequencing reaction was performed using PCR with the … reign the shakedownWeb24 jul. 2024 · ITS was amplified using primers ITS1F and ITS4R or ITS4R and ITS5F (White et al. 1990). The thermal cycle used for the amplification of all the loci was 5 min at 94 … procter and gamble pakistan addressWeb一种马尔尼菲篮状菌的分子鉴定方法，包括以下步骤：s1：引物确定：包括外围引物、扩增引物和测序引物；s2：pcr条件确定：根据引物的tm值确定；s3：样本dna提取：样本选择人体组织液；s4：巣式pcr扩增：巣式pcr扩增的反应体系为20µl体系；s5：琼脂糖凝胶电泳检测：琼脂糖凝胶电泳样品为s4获得的 ... procter and gamble pampers coupons