Hieff clone plus multi one step cloning kit

WebVazyme ClonExpress II One Step Cloning is a simple, fast, and high efficient cloning technology and enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert ... Web9 de dez. de 2024 · HR donor plasmids were constructed using the Hieff Clone Plus Multi One Step Cloning Kit (YEASEN, China). The G622A HR donor was constructed by ligating four fragments (a left arm, ...

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WebToo lazy to do cloning work? Empower your laziness with our Pro Ligation-free Cloning Kit! This bestselling kit offers:1) One-step cloning - no reliance on r... WebGenBuilder™ Cloning Kit: GenBuilder™ Plus Cloning Kit: 1: Unique need fulfilled by the kit: Multi-gene cloning for up to 6 fragments: Multi gene cloning for up to 12 fragments: 2: Cost per reaction: $7.90: $9.80: 3: Number of fragments that can be cloned into a linearized vector: 6: 12: 4: Cloning efficiency (insert was cloned into the ... high ankle shoes lowest price https://bodybeautyspa.org

Hieff Clone Plus Multi One Step Cloning Kit Cat No. 10912

Web28 de set. de 2024 · Hieff Clone Plus Multi One Step Cloning Kit (YEASEN, Shanghai, China) was used to develop co-expressing plasmids of pET-rrbdh-nox, pET-rrbdh-nox-vgb, pET-ssbdh-nox, and pET-ssbdh-nox-vgb according to the provided protocol. Web24 de abr. de 2024 · In summary, the multiple genes transformation increased the SF content in broccoli more than single gene introductions. The aim of this research was to improve sulforaphane content by introducing FMOGS–OX2, ... (Hieff Clone Plus Multi One Step Cloning Kit, Yeasen, shanghai, China). Webusing Hieff Clone Plus One Step Cloning Kit (Yeasen Biotech Co. Ltd.) and the vectors were transformed into EscherichiacoliDH5 cells.Sequenceswereconfirmedby DNA sequencing. The plasmids were extracted by Plasmid Maxi Preparation Kit or Plasmid Maxiprep Kit (Bey-otime, Shanghai, China), and subsequently transfected into high ankle sneakers black

Hieff CloneTM一步法同源重组快速定向克隆试剂盒 - 产品 ...

Category:Universal One Step Cloning Kit-Yeasen

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Hieff clone plus multi one step cloning kit

Novel crosstalk between ethylene‐ and jasmonic acid‐pathway ...

WebThe Hieff Clone™ Universal One Step Cloning Kit ensures efficient single- and multi-fragment cloning. A-B: Recombinant transformation plates. C: Electropherogram of … WebFigure 5. Single fragment cloning of 2290 bp was performed with the Hieff Clone™ Plus One Step Cloning Kit. A: line 1 is the linear vector, and line 2 is the target DNA …

Hieff clone plus multi one step cloning kit

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Web产品描述. Hieff Clone TM Plus Multi One Step Cloning Kit 是一款简便、快速、高效的 DNA 定向克隆产品，该试剂盒可以将 PCR 产物定向克隆至任何载体的任何位点 ，可高效克隆 50 bp-10 kb 片段。 将 载体线性化，并在插入片段正、反向 PCR 引物 5’ 端引入 15-25 bp 的线性化载体末端同源序列，使得插入片段 PCR 产物 ... WebHieff Clone® Plus One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任何载体的任何位点，可高效克隆50 bp-10 kb片段。 …

Web20 de mai. de 2024 · The purified PCR product was then cloned into the pCAMBIA1300-UBI-RBCS vector using the Hieff Clone® Plus One Step Cloning Kit. The D3 knockout vector was constructed using the CRISPR/Cas9 method. Based on the genomic sequence of D3 gene, two specific CRISPR target sites were designed in the protein-encodable … WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, …

WebItem Hieff Clone® Plus Multi One Step Cloning Kit; Company Yeasen Biotechnology Co., Ltd; Catalog Number 10912; This product is no longer available on Biocompare. … http://yeasen.com/assets/upload/file/20240817130924833.pdf

Web12 de ago. de 2024 · The full-length cDNA of OsEBF1 (LOC_Os06g40360) was amplified with KOD-plus DNA polymerase (Toyobo, Tokyo, Japan) using the primers EBF1OE-attB-F and EBF1OE-attB-R, and cloned into the p1301-35SNos vector using a Hieff-Clone™ Plus Multi One Step Cloning Kit (Yeasen, Beijing, China).

Web13 de abr. de 2024 · 高保真聚合酶（Hieff Canace Plus High-Fidelity DNA Polymerase，货号 10153ES60） 一步法克隆试剂盒（Hieff Clone ® Plus One Step Cloning Kit 一步 … high ankle sneakers for menWebIn-Fusion® HD EcoDry™ Cloning Kit User Manual (080318) takarabio.com Takara Bio USA, Inc. Page 3 of 14 I. Introduction In-Fusion HD EcoDry Cloning Kits are designed for fast and convenient, directional cloning of one or more fragments of DNA into any vector. The cornerstone of In-Fusion Cloning technology is our proprietary In-Fusion how far is iah from hobbyWebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, … high ankle sneakers for ladiesWebDescription. The GenBuilder Plus Cloning Kit enables seamless and one-step cloning of multiple DNA fragments into a linearized vector. The proprietary enzyme mix fuses together DNA fragments efficiently and precisely by recognizing 15-40 bp overlaps at the end of each fragment. The system allows cloning of DNA fragments into any linearized ... how far is iah airport to galveston porthigh ankle sneakersWebBasic Steps of Cloning. There are many approaches available for cloning DNA, most of which follow this basic workflow: Isolation of target DNA - typically referred to as an … how far is ibaraki from tokyoWebGostaríamos de lhe mostrar uma descrição aqui, mas o site que está a visitar não nos permite. how far is iad to dca airport