Cisplatin adducts

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August undefined, 2024

WebThe kinetics of bifunctional cisplatin adduct formation were studied with DNA in vitro and in cultured Chinese hamster ovary (CHO) cells. Prior to adduct measurements with AAS in … WebDec 19, 2024 · For instance, cisplatin forms mainly 1,2-intrastrand crosslinks (~80%) and rarely interstrand crosslinks (~1.5%) between two nucleobases in double-stranded DNA [21,22], whereas transplatin can hardly form intrastrand crosslinks but forms monofunctional adducts and interstrand crosslinks at a higher frequency than cisplatin.

Recognition of cisplatin adducts by cellular proteins

WebMay 8, 2014 · The masses of the platinum adducts following digestion and separation by HPLC were measured by mass spectrometry. Our results demonstrate that hydrated electrons induce damage to thymines as well as detachment of the cisplatin moiety from both guanines in the oligonucleotide. WebJul 1, 2008 · Although oxaliplatin and cisplatin form similar adducts on DNA, cisplatin is a poor substrate for OCT1 and OCT2 compared with oxaliplatin and is not active against colorectal cancer. These properties suggest that OCT-mediated uptake of oxaliplatin into cancer cells is a major determinant of its activity. csharp cross platform ui

Adduct-specific monoclonal antibodies for the measurement of cisplatin …

WebNov 7, 2016 · Cisplatin, one of the most widely used anticancer drugs, crosslinks DNA and ultimately induces cell death. However, the genomic pattern of cisplatin-DNA … WebThe synthesis and characterisation of novel metal-modified DNA precursors for fuel cell catalyst development are described. Material precursors in the form of metal–DNA … WebCisplatin is considered to be the most active platinum agent drug. Neoadjuvant (or induction) chemotherapy can be used for cervical cancer patients with locally advanced disease; this treatment is followed by radical surgery and/or radiation therapy. Cisplatin is considered to be the most active platinum agent drug csharp cryptostream

Human colon cancer cells surviving high doses of cisplatin or ... - PubMed

Recognition and repair of DNA-cisplatin adducts - PubMed

WebThe cytotoxicity of platinum compounds is thought to be determined primarily by their DNA adducts. Cisplatin and oxaliplatin are structurally distinct, but form the same types of adducts at the same sites on DNA. However, the DNA adducts are differentially recognized by a number of cellular proteins … WebCisplatin adducts are Guanine-Guanine (GG) DNA intrastrand cross links caused by the chemical compound Cisplatin. Cisplatin is used as a chemotherapy agent that contains … each web cameraWebOct 23, 2003 · Cisplatin is a neutral inorganic, square planar complex that reacts with DNA to induce its characteristic biological effects, which culminate in either repair of the DNA damage and cell survival... each weight

"WebIn nDNA, cisplatin-DNA adducts are removed and DNA is repaired by nucleotide excision repair (NER). Mitochondria do not have NER mechanisms for mtDNA. We demonstrate that cisplatin forms adducts with mtDNA in DRG neurons, and that this alters mtDNA replication and transcription. " - Cisplatin adducts

Cisplatin adducts

Cisplatin - Group 10: platinum anticancer agents - Pharmacy 180

WebChromatin will also contain drug-adducts on proteins and these will not be recognized at all. Most people doing immunohistochemical techniques for DNA modifications … WebIn general, combination of cisplatin with an antimetabolite, taxane, or topoisomerase inhibitor, can result in a modulation of platinum pharmacology on the DNA, for example, enhanced retention of the platinum-DNA adducts. These interactions are mostly sequence and cell type dependent.

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WebCisplatin (NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP) is an inorganic platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts in tumor cells. Cisplatin activates ferroptosis and induces autophagy. Solutions are best fresh-prepared. WebCisplatin is a major anticancer drug that kills cancer cells by damaging their DNA. Cancer cells cope with the drug by removal of the damages with nucleotide excision repair. We …

WebSep 5, 2011 · The recognition of inter- and intra-strand DNA adducts and the consequent generation of an apoptotic signal is often impaired in cisplatin-resistant cancer cells … WebCisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. Therefore, identifying novel biomarkers to improve the diagnosis and treatment of NSCLC is important. ... DDP binds to DNA and forms DNA-platinum adducts, which disrupt DNA synthesis and ...

WebApr 11, 2024 · HIGHLIGHTS who: Umit Aliyaskarova from the Universitu00e9 Nazarbayev University have published the paper: NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells, in the Journal: Scientific … Neil3-mediated proteasomal degradation facilitates the repair of cisplatin-induced dna … WebSep 24, 2024 · Cisplatin (CDDP) is the drug of choice against different types of cancer. However, tumor cells can acquire resistance to the damage caused by cisplatin, …

WebApr 9, 1999 · Cisplatin is well known as a DNA-damaging agent, and the specific adducts produced in DNA have been well characterized. However, the mechanism by which …

WebHuman colon cancer cells surviving high doses of cisplatin or oxaliplatin in vitro are not defective in DNA mismatch repair proteins These results indicate that high-level resistance of human colon cancer cells to high doses of cisplatin and oxaliplatin does not seem to be related to acquired defects in the DNA MMR proteins. each wellWebApr 12, 2012 · Cisplatin exerts anticancer effects via multiple mechanisms, yet its most prominent (and best understood) mode of action involves the generation of DNA lesions followed by the activation of the DNA damage response … each wild idea the joy of photographyWebJan 1, 2001 · Pt adducts on DNA extracted from cells that had been treated with cisplatin were less immunoreactive than DNA treated with cisplatin in vitro, but after heating the immunoreactivity increased such that 50% inhibition in the assay was caused by 2 … each week of navy boot campWebJul 18, 2007 · One potential predictive marker is the formation of cisplatin-DNA adducts, which are formed when cisplatin reacts with the cellular DNA by binding to nucleotides. … each wild programWebDespite intense research efforts, no approved therapy is yet available. Cisplatin is a chemotherapeutic drug but induces acute tissue injury. Oleuropein (OLE) is a major … csharp cs8601WebThere was also a statistically significant inverse correlation between cisplatin/DNA adduct formation and the IC50 for cisplatin in these cell lines. (P = 0.0004, R2 = 0.67). In … each wild idea writing photography historyWebDetection and characterization of an individual cisplatin adduct on a single DNA molecule is a demanding task. We explore the characteristic features of cisplatin adducts in the … each will be assigned a different color