Webdesigned to develop and evaluate the chitosan-graft-polyethylenimine (CP)/DNA nanoparticles as novel non-viral gene vectors for gene therapy of osteoarthritis. WebThere are two main approaches for gene delivery using viral and non-viral gene transfer systems for treating articular cartilage defects in OA, or following joint trauma. The first is …
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WebAug 31, 2024 · Although gene delivery systems can be viral or non-viral, non-viral gene delivery materials are being specifically developed that could deliver the gene as a safer … WebJan 2, 2014 · The present study was designed to develop and evaluate the chitosan-graft-polyethylenimine (CP)/DNA nanoparticles as novel non-viral gene vectors for gene …
WebDec 17, 2024 · To construct a novel non-viral vector loaded with growth and differentiation factor-5 (GDF-5) plasmid using chitosan, hyaluronic acid, and chondroitin sulfate for osteoarthritis (OA) gene therapy. Methods Nano-microspheres (NMPs) were prepared by mixing chitosan, hyaluronic acid, and chondroitin sulfate. WebSep 24, 2024 · 2.1. Solubility. Chitosan is produced by deacetylation of chitin; in this process, some N-acetylglucosamine moieties are converted into glucosamine units.The presence of large amounts of protonated -NH 2 groups on the chitosan structure accounts for its solubility in acid aqueous media since its pKa value is approximately 6.5 [].When …
WebIntroduction. Cartilage injury, a common clinical problem, can be caused by trauma, 1 osteoarthritis, 2 rheumatoid arthritis, 3 and exfoliative chondritis. 4 Articular cartilage is … WebJun 1, 2024 · Modifying chitosan with target ligands is a promising method for the targeted delivery of genes by delivery systems based on chitosan. Proteins, peptides, aptamers, carbohydrates, and small molecules are common ligands used to realize target delivery in gene vectors based on chitosan.
WebSep 14, 2024 · Chitosan is a derivation from chitin. Its favorable biological properties including biocompatible, biodegradable, and non-toxicity, make chitosan as a promising drug delivery carrier. In addition, chitosan is a water-soluble polymer which makes the process of preparation easier and milder.
WebChitosan is a fibrous compound derived from chitin, which is the second most abundant natural polysaccharide and is produced by crustaceans, including crabs, shrimps, and lobsters. Chitosan has all of the important medicinal properties, including biocompatibility, biodegradability, and hydrophilicity, and it is relatively nontoxic and cationic in nature. … jequitiba barWebFeb 15, 2024 · 1. Introduction. Osteoarthritis (OA) is a progressive and degenerative joint disease that causes articular cartilage damage, synovial inflammation, and subchondral bone remodeling [1].World Health Organization (WHO) estimates that 9.6% of men and 18.0% of women aged over 60 years have symptomatic OA [2].OA affects life quality of … jequitibáWebThingreila Muinao, ...Mintu Pal, in Polysaccharide Carriers for Drug Delivery, 2024. 15.4.2.9 Chondroitin sulfate. Chondroitin sulfate is a natural water soluble polymer comprising of repeating disaccharide units of d-glucuronic acid and N-acetylgalactosamine, sulfated at either 4- or 6-positions.While cross-linked chondroitin sulfates may show less hydrophilic … jequiti avon boticarioWebChitosan–pDNA nanoparticle characteristics determine the transfection efficacy of gene delivery to human mesenchymal stem cells E Malakooty Poor, M Baghaban Eslaminejad, N Gheibi, F Bagheri, ... Artificial cells, nanomedicine, and biotechnology 42 (6), 376-384 , 2014 lama meetingWebJan 28, 2024 · A The optical images of the gelation progress. The mixture of N-chitosan and ADH presented in the sol state at room temperature, and it experienced sol-gel … jequitiba em bh mgWebJan 1, 2024 · Thermoresponsive DDS, chitosan-modified MoS 2 (molybdenum disulfide), was shown to release a small molecule drug, dexamethasone (Dex) ... is most commonly employed osteoarthritis therapeutic for gene delivery; currently there are two ongoing trials for intra-articular delivery using an AAV (NCT02790723) and an HDAV … jequitiba araucoWebApr 12, 2024 · Chitosan is a naturally occurring polysaccharide that is derived from chitin, a major component of the exoskeletons of crustaceans such as shrimp, crab, and lobster. It has been gaining attention in the field of biotechnology and biomedicine due to its unique properties and potential applications. Chitosan is a linear polysaccharide that consists of … lama meier